Eating after midnight raise cravings

Obesity affects around 42 percent of adult Americans in the United States, which raises the risk of having chronic diseases such as diabetes, cancer, and other illnesses.

Few studies have thoroughly examined the simultaneous effects of late eating on the three main players in body weight regulation and thus obesity risk: regulation of calorie intake, the number of calories you burn, and molecular changes in fat tissue.

Popular healthy diet mantras discourage midnight snacking, but few studies have investigated the effects of late eating on all three players simultaneously. Researchers from Brigham and Women's Hospital, a founding institution of the Mass General Brigham healthcare system, discovered in a recent study that the timing of meals has a big impact on our metabolism, hunger, and biochemical pathways in adipose tissue. Cell Metabolism has reported their findings.

According to senior author Frank A. J. L. Scheer, Ph.D., Director of the Medical Chronobiology Program in Brigham's Division of Sleep and Circadian Disorders, "We sought to test the processes that may explain why late eating raises obesity risk." "Previous studies conducted by us and others have demonstrated that eating in the evening increases the risk of becoming obese, increases body fat, and hinders the success of weight loss. We were curious as to why."

"In this study, we asked, 'Does the time that we eat matter when everything else is kept consistent?'" said first author Nina Vujovic, Ph.D., a researcher in the Medical Chronobiology Program in the Brigham's Division of Sleep and Circadian Disorders. "And we found that eating four hours later makes a significant difference for our hunger levels, the way we burn calories after we eat, and the way we store fat."

16 patients with a body mass index (BMI) in the overweight or obese range were the subject of a study by Vujovic, Scheer, and their colleagues. Each participant did two lab protocols: one with an exact early meal schedule and the other with the same meals timed exactly four hours later in the day. Participants kept set sleep and wake times in the final two to three weeks before beginning each in-lab regimen, and in the final three days before entering the lab, they closely adhered to similar meals and meal times at home.

Participants frequently kept track of their hunger and appetite in the lab, gave us numerous times during the day tiny blood samples, and had our researchers assess their body temperature and energy expenditure. During laboratory testing in both the early and late eating protocols, researchers took biopsies of adipose tissue from a subset of participants to enable a comparison of gene expression patterns/levels between these two eating conditions. This allowed them to measure how eating time affected molecular pathways involved in adipogenesis, or how the body stores fat.

The findings showed that eating later had significant impacts on the hunger and appetite-controlling chemicals leptin and ghrelin, which affect our desire to eat. Leptin levels, which indicate fullness, were specifically lower over the course of 24 hours in the late meal condition compared to the early feeding conditions. Participants who ate later burnt calories more slowly and showed altered gene expression in their adipose tissue, which promotes greater adipogenesis and decreased lipolysis. These results reveal convergent physiological and molecular mechanisms that underlie the association between eating later in the day and a higher risk of obesity.

According to Vujovic, these results not only support a substantial body of evidence that suggests eating later may raise the risk of becoming obese, but they also provide new insight into how this can happen. Researchers were able to identify changes in the various control systems involved in energy balance, a sign of how our bodies use the food we eat, by using a randomized crossover study and strictly controlling for behavioral and environmental factors such as physical activity, posture, sleep, and light exposure.

To make their findings more applicable to a larger population, Scheer's team plans to increase the proportion of female participants in subsequent trials. Although there were only five female participants in this study cohort, the study was designed to control for the menstrual phase, which reduced confounding but made it more challenging to get women to participate. In the future, Scheer and Vujovic want to learn more about how the link between mealtime and nighttime affects energy balance.

"This study shows the impact of late versus early eating. Here, we isolated these effects by controlling for confounding variables like caloric intake, physical activity, sleep, and light exposure, but in real life, many of these factors may themselves be influenced by meal timing," said Scheer. "In larger scale studies, where tight control of all these factors is not feasible, we must at least consider how other behavioral and environmental variables alter these biological pathways underlying obesity risk."